Coding

Part:BBa_K2307004:Design

Designed by: Marguerite Batsale   Group: iGEM17_Bordeaux   (2017-10-26)


unc-60 RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 335
    Illegal XhoI site found at 63
    Illegal XhoI site found at 290
    Illegal XhoI site found at 1024
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFP was merged with Exons E1,E2A and Intron 1A of the WT gene and was placed in the C-terminal of the protein.


Source

This reporter was designed by Hidehito Kuroyanagi that can be found in the article "Muscle-Specific Splicing Factors ASD-2 and SUP-12 Cooperalively Switch Alternative Pre-mRNA Processing Patterns of the ADF/Cofilin Gene in Caenorhabditis elegans" and published in october 2012 in PLOS Genetics.

References